yqeL [2017-8-11 16:0:3]

ribosomal silencing factor

locus: BSU25620

pI: 4.37

mw: 13.16 kDa

protein length: 118 aa

gene length: 354 bp

function: translation

product: ribosomal silencing factor

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]

Gene

Coordinates: 2,642,841 → 2,643,197

The protein

Catalyzed reaction/ biological activity

• silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairs subunit joining [Pubmed|22829778]

Protein family

• Iojap family (according to Swiss-Prot)

Structure

• [PDB|2O5A] (from B. halodurans, 65% identity)

'''[SW|Interactions]:

• [protein|2BE91EEB70A1961D3BBE0603648E64E86CD0B708|RplN]-[protein|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|RsfS] [Pubmed|22829778], the interaction serves to silence [SW|translation] [Pubmed|22829778]

Additional information

• the protein is enriched in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA] [Pubmed|23700310]

Expression and Regulation

Operons

• genes: [gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|rsfS]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C500 (yqeL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2498 NBRP B. subtilis, Japan]

References

• 22829778,23700310,22383849